Fitting two- and three-site binding models to isothermal titration calorimetric data
نویسندگان
چکیده
منابع مشابه
Metal ions binding study on human growth hormone by isothermal titration calorimetric method
The interaction of hGH with some metal ions ( ) at 27°C in NaC1 solution, 50 mM was studied using Isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for binding of all these metal ions, expect . The intrinsic association equilibrium constants () are not very different for and , and also their molar enthalpies of binding (KJ/mol for and KJ/mo...
متن کاملmetal ions binding study on human growth hormone by isothermal titration calorimetric method
the interaction of hgh with some metal ions ( ) at 27°c in nac1 solution, 50 mm was studied using isothermal titration calorimetry. there is a set of three identical and non-interacting binding sites for binding of all these metal ions, expect . the intrinsic association equilibrium constants () are not very different for and , and also their molar enthalpies of binding (kj/mol for and kj/mo...
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Microcalorimetric and spectropolarimetric titrations were used to investigate micellization of cationic surfactants and binding of netropsin to dodecameric DNA duplex. For description of both processes, model functions containing linear and non-linear parameters were derived. Model analysis was based on a weighted (multi)linear regression and a standard »Simplex« procedure. Close investigation ...
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Isothermal titration calorimetry (ITC) is now routinely used to directly characterize the thermodynamics of biopolymer binding interactions and the kinetics of enzyme-catalyzed reactions. This is the result of improvements in ITC instrumentation and data analysis software. Modern ITC instruments make it possible to measure heat effects as small as 0.1 microcal (0.4 microJ), allowing the determi...
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The hammerhead ribozyme undergoes a well-defined two-stage conformational folding process, induced by the binding of magnesium ions. In this study, we have used isothermal titration calorimetry to analyze the thermodynamics of magnesium binding and magnesium ion-induced folding of the ribozyme. Binding to the natural sequence ribozyme is strongly exothermic and can be analyzed in terms of seque...
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ژورنال
عنوان ژورنال: Methods
سال: 2015
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2014.11.018